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珊瑚與渦鞭毛藻共生現象之細胞與分子生物學
研究計劃資料
計畫名稱 : 珊瑚與渦鞭毛藻共生現象之細胞與分子生物學
研究人員 : 陳啟祥(計劃主持人)、王立雪、彭紹恩、李幸慧、江佩倫
子計畫 :
1. 珊瑚與渦鞭毛藻共生現象之細胞與分子生物學 (陳啟祥)
2. 珊瑚細胞與共生藻間細胞週期的相互調控機制 (王立雪)
3. 刺絲胞動物與渦鞭毛藻之辨識及建立共生的機轉 (彭紹恩)
4. 探討胞內共生特有的分子及其特性 (李幸慧)
5. 藻細胞油體的鑑定及功能性分析 (江佩倫)

計劃說明 :
具有光合作用能力之共生藻與海洋刺絲胞動物如海葵、珊瑚間的胞內共生現象,是維繫珊瑚礁生態系之高物種歧異度及高生產力的主因。共生藻是經由胞噬作用進入宿主細胞,經由一連串的辨識過程,進而形成穩定的胞內共生現象。海洋刺絲胞動物及共生藻細胞間具有物質交換系統,能將共生藻的光合作用產物如甘油及葡萄糖運送給宿主細胞使用,而宿主細胞則將呼吸作用及含氮代謝廢物等供給共生藻做為養分。當環境發生劇烈變化如溫度的驟升或驟降、水質酸化或濁度增加以及人為的破壞均會影響共生現象,造成珊瑚的白化、死亡,進而影響珊瑚礁生態系的平衡。本研究團隊利用分子細胞學、蛋白質體學、質譜學及顯微影像學等來研究細胞膜蛋白質、脂質循環、胞器分布、細胞分裂、光合作用及相關分子結構在胞內共生現象中之調控,並針對此一複雜之動植物間的交互作用進行深入研究,以期有系統地了解胞內共生之機轉,使此方面的研究深入分子層面並臻於世界領導地位,進一步藉此開發海洋生物免疫學、代謝體學及細胞學等方面的重要研究領域,並提供教學訓練的機會,期有助於海洋生物細胞學及醫學研究的前瞻進展暨人才培育。

研究成果 :
Chen, H.K., Sung, S.N., Wang, L.H., Mayfield, A.B., Fang, L.S. Chen, U.W.N. and Chen, C.S. (2015) A compartmental comparison of major lipid species in a coral-Symbiodinium endosymbiosis: evidence that the coral host regulates lipogenesis of its cytosolic lipid bodies. PLOS One (DOI:10.1371/journal.pone. 0132519)
Wang, L.H., Chen H.K., Jhu, C.S., Cheng, J.O., Fang, L.S. and Chen, C.S. (2015) The different strategies of energy storage in cultured and freshly isolated Symbiodinium sp. J. Phycol.
Jiang, P.L., Pasaribu B., and Chen C.S. (2014) Nitrogen-Deprivation Elevates Lipid Levels in Symbiodinium spp. by Lipid Droplet Accumulation: Morphological and compositional analyses. PLOS ONE (DOI:10.1371/journal.pone.0087416).
Li, H.H., Huang, Z.Y., Ye, S.P., Lu, C.Y., Cheng, P.C., Chen, S.H. and Chen, C.S. (2014) Membrane labeling of coral gastrodermal cells by biotinylation: the proteomic identification of surface proteins involving Cnidaria-dinoflagellate endosymbiosis. PLoS ONE (DOI: 10.1371/journal.pone.0085119).
Pasaribu, B., Lin, I.P., Tzen, J.T.C., Jauh, G.Y., Fan, T.Y., Ju, Y.M., Cheng, J.O., Chen, C.S. and Jiang, P.L. (2014) SLDP: A novel protein related to caleosin is associated with the endosymbiotic Symbiodinium lipid droplets from Euphyllia glabrescens. Marine biotechnology. 16: 560-571.
Weng, L.C., Pasaribu, B., Lin, I.P., Tsai, C.H., Chen, C.S. and Jiang, P.L. (2014) Nitrogen deprivation induces lipid droplet accumulation and alters fatty acid metabolism in symbiotic dinoflagellates isolated from Aiptasia pulchella. Scientific Reports 4: 5777; (DOL:10.1038/srep05777).
Mayfield, A.B., Wang, Y.U., Chen, C.S., Lin, Y.C. and Chen, S.H. (2014) Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures. Molecular Ecology. 23(23):5816-5830.
Sogin, E.M., Anderson, P., Williams p., Chen, C.S. and Gates R.D. (2014) Application of 1H-NMR metabolomic profiling for reef-building corals. PLoS ONE (DOI: 9(10):e111274).
Mayfield, A.B., Chen, Y.H., Dai, C.F. and Chen, C.S. (2014) The effect of temperature on gene expression in the Indo-Pacific reef-building coral Seriatopora hystrix: insight from aquarium studies in southern Taiwan. International Journal of Marine Science 4(50):1-22.
Mayfield, A.B., Fan, T.Y. and Chen, C.S. (2014) Real-time PCR-based gene expression analysis in the model reef-building coral Pocillopora damicornis: insight from a salinity stress study. Platax 10:1-19.
Mayfield, A.B., Hsiao Y.Y., Chen H.K. and Chen C.S. (2014) Rubisco expression in the dinoflagellate Symbiodinium sp. is influenced by both photoperiod and endosymbiotic lifestyle. Marine Biotechnology 16:371-381.
Mayfield, A.B., Fan, T.Y., and Chen C.S. (2013) Physiological acclimation to elevated temperature in a reef-building coral from an upwelling environment. Coral Reefs. 32(4):909-921.
Mayfield, A.B., Chen, M.N., Meng, P.J., Lin, H.J., Chen C.S. and Liu P.J. (2013) The physiological response of the reef coral Pocillopora damicornis to elevated temperature: results from coral reef mesocosm experiments in Southern Taiwan. Marine Environmental Research 86:1-11.
Putnam, H.M., Mayfield, A.B., Fan, T.Y., Chen C.S. and Gates R.D. (2013) The Physiological and molecular responses of larvae from the reef-building coral Pocillopora damicornis expose to near-future increases in temperature and pCO2. Marine Biology 160(8):2157-2173.
Lin, C., Wang, L.H., Meng, P.J., Chen, C.S. and Tsai S. (2013) Lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts. PLOS One 8(2): e57823.
Wang, L.H., Lee, H.H., Fang, L.S., MayField, A.B. and Chen, C.S. (2013) Fatty acid and phospholipid syntheses are prerequisites for the cell cycle of Symbiodinium and their endosymbiosis within sea anemones. PLOS One 8(8):e72486.
Yost, D.M., Wang, L.H., Fan, T.Y., Chen, C.S., Lee, R.W., Sogin, E. and Gates, R.D. (2013) Diversity in skeletal architecture influences biological heterogeneity and Symbiodinium habitat in corals. Zoology 116:262-269
Mayfield A.B., Fan T.Y. and Chen C.S. (2013) The impacts of ex situ transplantation on the physiology of the Taiwanese reef-building coral Seriatopora hystrix. Marine Biology. Article ID 569361.
Chen, W.N.U., Kang, H.J., Weis, V.M., Mayfield, A.B., Jiang, P.L., Fang, L.S., Chen, C.S. (2012) Diel rhythmicity of lipid-body formation in a coral-Symbiodinium endosymbiosis. Coral Reefs, 31: P.521-P.534.
Chen, C.S., Yeh, S.P., Wang, L.H., and Chen, U.W.N. (2012) Increased susceptibility of algal symbionts to photo-inhibition resulting from perturbation of coral gasterodermal membrane trafficking. Sci.China Life Sci. 55:599-611.
Lin, C., Wang, L.H., Fan T.Y. and Kuo, F.W. (2012) Distribution of lipid content and lipid composition during the oocyte development of two gorgonian coral species (Junceella juncea and Junceella fragilis) in relation to low temperature preservation. PLOS One 7: e38689.
Peng, S.E., Chen, C.S., Song, Y.F., Huang, H.T., Jiang, P.L., Chen, W.N.U., Fang. L.S. and Lee, Y.C. (2012) Assessment of metabolic modulation in free-living versus endosymbiotic Symbiodinium using synchrotron radiation-based infrared microspectroscopy. Biology letter, 23: P.434-P.437.
Mayfield, A.B., Chan, P.S., Putnam, H.M., Chen, C.S. and Fan, T.Y. (2012) The effects of a variable temperature regime on the physiology of the reef-building coral Seriatopora hystrix: results from a laboratory-based reciprocal transplant. The Journal of Experimental Biology 215(23):4183-4195.
Peng, S.E., Chen, W.N.U., Chen, H.K., Lu, Y.C., Mayfield, A.B. and Chen C.S. (2011) Lipid bodies in coral-dinoflagellate endosymbiosis: proteomic and ultrastructural studies. Proteomics 11:3540-3555.
Mayfield, A.B., Wang, L.H., Tang, P.C., Fan, T.Y., Hsiao, Y.Y., Tsai, C.L., Chen, C.C. 2011. Assessing the impacts of experimentally elevated temperature on the biological composition and molecular chaperone gene expression of a reef coral. PLoS One 6:e26529.
Mayfield, A.B., Wang, L.H., Tang, P.C., Fang, T.Y., Hsiao, Y.Y., Tsai, C.L. and Chen, C.S. (2011) Assessing RNA, DNA, and protein-level responses to experimentally elevated temperatures in the reef-building coral Seriatopora hystrix. PLoS ONE 6(10): e26529.
Peng S.E., Wang, Y.B., Wang, L.H., Chen, W.N.U., Lu, C.Y., Fang, L.S. and Chen, C.S. (2010) Proteomic analysis of symbiosome membranes in Cnidarian-dinoflagellate endosymbiosis. Proteomics 10:1002-1016.
Mayfield, A.B., Hsiao, Y.Y., Fang, T.Y., Chen, C.S. and Gates, R.D. (2010) Evaluating the temporal stability of stress-activated protein kinase and cytoskeleton gene 1 expression in the Pacific reef corals Pocillopora damicornis and Seriatopora hystrix. J. Exp. Mar. Biol. Ecol. 395:215-222.
Peng, S.E., Wang Y.B., Wang L.H., Chen, W.N.U., Lu, C.Y., Fang L.S. and Chen, S.C. (2009) Proteomic analysis of symbiosome membranes in Cnidaria-dinoflagellate endosymbiosis. Proteomics 10:1002-1016.
Huang, H.J., Chang, C.Y., Peng, S.E., Wang, L.H. and Chen, S.C. (2009) An improved method of transmission electron microscopy to visualize the plasma membrane biotinylation of coral symbiotic gastrodermal cells. Platax 6:47-56.
Hsiao, Y.-Y., L.-H. Hou and C.-S. Chen* (2009) Diel RuBisCO expression in symbiotic dinoflagellate Symbiodinium sp. Platax 6: 69-81.
Wang, L.H., Shiea, J., Yeh, S.P., Twan, W.H., Chen, W.N.U., Huang, H.J., Chang, C.Y., Kang, H.J., Fang, L.S. and Chen, S.C. (2009) Increased susceptibility to photoinhibition by disruption of host gastrodermal membrane trafficking during cnidaria-dinoflagellate endosymbiosis. Biological Bulletin
Luo, Y.J., Wang, L.H., Chen, W.N.U., Peng, S.E., Tzen, J.T.C., Hsiao, Y.Y., Huang, H.J., and Chen, S.C. (2009) Ratiometric imaging of gastrodermal lipid bodies in coral-dinoflagellate endosymbiosis. Coral Reefs 28:289-301
Wang, L.H., Liu, Y.H., Lin, S.F., Fang L.S., and Chen, S.C. (2008) Cell cycle propagation is driven by light-dark stimulation in a cultured symbiotic dinoflagellate isolated from corals. Coral Reefs 27:823-835
Peng, S.E., Luo, Y.J, Huang, H.J., Lee I.T.,, Hou, L.H., Chen, W.N.U., Fang L.S. and Chen, S.C. (2008) Isolation of tissue layers in hermatypic corals by N-acetylcysteine: morphological and proteomic examinations. Coral Reefs 27:133-142
Huang, H.J., Wang, L.H., Chen, W.N.U, Fang L.S. and Chen, S.C. (2008) Developmentally regulated localization of endosymbiotic dinoflagellates in different tissue layers of coral larvae. Coral Reefs 27:365-372
Huang, H.J., Wang, L.H., Peng, S.E., Hsiao, Y.C., Chang, C.Y., Fang L.S. and Chen, S.C. (2007) New cell model for endosymbiosis research: spontaneous dissociation of endoderm cells based on the tissue polarity in coral. Platax 4:9-25
Chen, S.C., Yeh, C.C., Lin, H.P. and Fang L.S. (2005) The use of a fluorescent membrane probe to identify zooxanthellae in hospite among dissociated endoderm cell culture from coral. Protoplasma 226:175-179
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