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專長:

低溫生物學

遺傳保種學

分子生物學

電話:08-8825036

Email:chiahsin@nmmba.gov.tw

國立海洋生物博物館研究人員

個人研究著作

東華大學教師個人基本資料

學歷:

英國貝德弗特大學自然應用科學系 博士

 

經歷:

2015-迄今, 國立東華大學海洋生物科技研究所副教授

2013-迄今, 國立海洋生物博物館副研究員

2010-2015, 國立東華大學海洋生物科技研究所助理教授

2014, 泰國Chulalongkorn University海洋科學系訪問研究員

2009-2013, 國立海洋生物博物館助理研究員

2009-迄今, Platax 海洋生物學刊主編

2012, 僑務委員會馬來西亞水產養殖輔導講師

2011, 教育部海洋知識系列演講講師

2009, 南極研究探堪

2006-2008, 英國倫敦大學生物科學系訪問學人

2001-2009, 國立海洋生物博物館研究助理  

 

 

研究興趣:

珊瑚組織、細胞、生殖細胞及胚胎之冷凍保存

冷凍保存對生物體基因之影響

冷凍基因庫

水生生物遺傳保種及育種

 

進行中研究主題:

1 Development of cryopreservation techniques for coral oocytes
2 Studies on mitochondrial activity, membrane potential and distribution in coral oocytes after cryopreservation
3 Establishing a comprehensive specimen coral cryobank of cell lines and tissue explants
 

 

SCI Journal Papers


  1. V. Viyakarn, S. Chavanich, G. Chong, S.Tsai, C. Lin* (2017) Cryopreservation of sperm from the coral Acropora humilis. Cryobiology https://doi.org/10.1016/j.cryobiol.2017.10.007 (In Press). (SCI)
  2. S. Tsai, G. Chong, P.J. Meng, C. Lin* (2017) Sugars as supplemental cryoprotectants for marine organisms. Reviews in Aquaculture; doi:10.1111/raq.12195 (SCI)
  3. G. Chong, F.W. Kuo, S. Tsai, C. Lin* (2017) Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium. Scientific Reports 7:39396; doi:10.1038/srep39396 (SCI)
  4. G. Chong, S. Tsai, C. Lin* (2016) Cryopreservation and its molecular impacts on microorganisms. J. Fish. Soc. Taiwan, 43(4), 263-272
  5. G. Chong, S. Tsai, C. Lin* (2016) Factors responsible for successful cryopreservation of algae. J. Fish. Soc. Taiwan, 43(3), 153-162
  6. S. Tsai, W.C. Chang., C. Lin* (2016) Ultrastructural observation of oocytes of six types of stony corals. Tissue and Cell 48, 349–355 (SCI)
  7. S. Tsai, V. Yang, C. Lin* (2016) Comparison of the cryo-tolerance of vitrified gorgonian oocytes. Scientific Reports 6, 23290; doi:10.1038/srep23290 (SCI)
  8. G. Chong, S. Tsai, L.H. Wang, C.Y. Huang, C. Lin* (2016) Cryopreservation of the gorgonian endosymbiont Symbiodinium. Scientific Reports. 6, 18816; doi: 10.1038/srep18816. (SCI)
  9. S. Tsai, W. Yen, S. Chavanich, V. Viyakarn, C. Lin* (2015). Development of cryopreservation techniques for gorgonian (Junceella juncea) oocytes through vitrification PLoS ONE doi: 10.1371/journal.pone.0123409. (SCI)
  10. S. Tsai, P. Thongpooe, F.W. Kuo, C. Lin(2015). Impacts of low temperature preservation on mitochondrial DNA copy number in oocytes of the hard coral Echinopora sp. Mitochondrial DNA doi: 10.3109/19401736.2015.1036254. (SCI)
  11. P. Kuanui, S. Chavanich*, V. Viyakarn, M. Omori and C. Lin (2015). Effects of temperature and salinity on survival rate of cultured corals and photosynthetic efficiency of the zooxanthellae in coral tissues. Ocean Sci 50(2), 263-268 doi.org/10.1007/s12601-015-0023-3 (SCI)
  12. L.H. Wang, C Lin*, C.Y. Huang, S. Tsai* (2015) Studies on lipid content and composition in banded coral shrimp ( Stenopus hispidus) embryos. J Crustacean Biol 35(5), 622-626 (SCI). * contributed equally as corresponding authors
  13. S. Tsai, Y. Jhuang, E, Spikingsc, P.J. Sung, C. Lin* (2014) Ultrastructural observation on the oogenesis of gorgonian coral (Junceella juncea). Tissue Cell 46, 225-232 (SCI)
  14. C. Lin*, F.W. Kuo, S. Chavanich, V. Viyakarn (2014) Membrane lipid phase transition behavior of oocytes from three gorgonian corals in relation to chilling injury. PLoS ONE 9(3): e92812. doi:10.1371/journal.pone.0092812. (SCI)
  15. S. Tsai, L.H. Wang, C. Lin. (2014) The chloroplast large subunit 23sDNA sequence of Symbiodinium fresh isolated from Junceella fragilis. NCBI. GenBank accession no. KP201138.
  16. S. Tsai, V. Kuit, Z.G. Lin and C. Lin* (2014). Application of a functional marker for the effect of cryoprotectant agents on gorgonian coral (Junceella juncea and J. fragilis) sperm sacs. CryoLetters 35 (1), 1-7 (SCI)
  17. S. Tsai, J.C. Chen, E. Spikings, J.J Li and C. Lin* (2014). Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes. Mitochondrial DNA doi:10.3109/19401736.2013.855734 (SCI)
  18. C. Lin, L.H. Wang, P.J. Meng, C.S. Chen and S. Tsai* (2013) Lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts. PloS ONE 8(2): e57823. doi:10.1371/journal.pone.0057823 (SCI)
  19. C. Lin, C.C Han and S. Tsai* (2013). Effect of thermal injury on embryos of banded coral shrimp (Stenopus hispidus) under hypothermal conditions. Cryobiology 66, 3-7 (SCI)
  20. C. Lin*, L.H. Wang, T.Y. Fan and F.W. Kuo (2012) lipid content and composition during the oocyte development of two gorgonian coral species (Junceella juncea and Junceella fragilis) in relation to low temperature preservation. PloS ONE 7(7): e38689. doi:10.1371/journal.pone.0038689 (SCI)
  21. C. Lin and S. Tsai* (2012). The effect of chilling and cryoprotectants on hard coral (Echinopora spp.) oocytes during short-term low temperature preservation. Theriogenology 77, 1257-1261 (SCI)
  22. S. Tsai and C. Lin* (2012).Development of an enzymatic method for individual separation of banded coral shrimp (Stenopus hispidus) embryos. Aquacult. Res. 43(10), 1509-1514 (SCI)
  23. C. Lin, F.W. Kuo, T. Zhang and S.Tsai*. Gorgonian coral (Junceella juncea and Junceella fragilis) oocyte chilling sensitivity in the context of adenosine triphosphate response , in: Alcohols—Advances in Research and Application: 2012 Edition
  24. S. Tsai and C. Lin* (2012). Advantages and applications of cryopreservation in fisheries science. Braz. Arch. Biol. Technol. 55(3), 425-433 (SCI)
  25. C. Lin, F.W. Kuo, T. Zhang and S.Tsai* (2011). Gorgonian coral (Junceella juncea and Junceella fragilis) oocyte chilling sensitivity in the context of adenosine triphosphate response. CryoLetters 32(2)141-147(SCI)
  26. S. Tsai, E. Spikings I.C. Haung and C. Lin* (2011) Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants. CryoLetters 32(1)1-12 (SCI)
  27. C. Lin and S. Tsai* (2011). The effect of cryopreservation on DNA damage, gene expression and protein abundance in vertebrate. Ital. J. Anim. Sci. 11, 119-122 (SCI)
  28. S. Tsai, E. Spikings, F.W. Kuo and C. Lin* (2010). Use of an adenosine triphosphate assay, and simultaneous staining with fluorescein diacetate and propidium iodide, to evaluate the effects of cryoprotectants on hard coral (Echinopora sp.) oocytes. Theriogenology 73, 605-611 (SCI)
  29. S. Tsai, W.C. Liu and C. Lin* (2010). An efficient approach for cryopreservation of the king grouper (Epinephelus lanceolatus) spermatozoa. Platax 7, 56-66.
  30. S. Tsai, E. Spikings and C. Lin* (2010). Effects of the controlled slow cooling procedure on freezing parameters and ultrastructural morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) sperm. Aquat. Living Resour. 23, 119-124 (SCI)
  31. S. Tsai and C. Lin* (2009). Effects of cryoprotectant on the embryos of banded coral shrimp (Stenopus hispidus), preliminary studies to establish freezing protocols. CryoLetters 30(5), 373-381 (SCI)
  32. C. Lin and S. Tsai* (2009). Factors affecting cryopreservation of cells. Platax 6, 93-106
  33. C. Lin, E. Spikings, T. Zhang and D.M. Rawson (2009) Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryo and blastomeres Cryobiology 59, 42-47 (SCI)
  34. C. Lin, E. Spikings, T. Zhang and D.M. Rawson (2009) Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres Theriogenology 71(7), 1147-55 (SCI)
  35. C. Lin, T. Zhang and D.M. Rawson (2009) Cryopreservation of zebrafish (Danio rerio) blastomeres by controlled slow cooling. CryoLetters 30(2), 132-141 (SCI)
  36. J.C.Chen and C. Lin (2001) Toxicity of copper sulfate for survival, growth, molting and feeding of juveniles of the tiger shrimp, Penaeus Monodon Aquaculture 192 55-65 (SCI)
  37. C. Lin and J.C. Chen (2001) Hemolymph oxyhemocyanin and protein levels and acid-base balance in the tiger shrimp Penaeus Monodon exposed to copper sulfate J. World Aquaculture Soc 32, 335-341 (SCI)

 

國際會議報告


 

  1. G. Chong, S. Tsai, P. J. Meng, C. Lin (2017) A strategy for coral conservation in response to the global warming and climate change: the Frozen Ark. 2017 Ocean Science Conference. May. Taiwan
  2. Y.H Goh, S Tsai, L.H. Wang, C Lin (2016) Cryopreservation of oocytes of the gorgonian coral Junceella fragillis using a controlled, slow- freezing protocol. The 7th International symposium for marine biotechnology. September. Taiwan
  3. C. Juri, S. Tsai, L.H. Wang, C. Lin (2016) Successful cryopreservation of a thermotolerant lineage of the coral reef dinoflagellate Symbiodinium. The 7th International symposium for marine biotechnology. September. Taiwan
  4. S. Tsai, V. Yang, C. Lin (2016) Comparison of the cryo-tolerance of vitrified gorgonian oocytes. The 23rd pacific science congress. June. Taiwan
  5. C. Lin, S. Tsai (2015) Thermal injury on mitochondria of coral oocytes at hypothermic conditions. The 6th International symposium for marine biotechnology. September. Taiwan
  6. G. Chong, L.H. Wang, S. Tsai, C. Lin (2015) Use of adenosine triphosphate bioassay to assess the effects of cryoprotectants on symbiotic algae of Junceella fragilis. The 6th International symposium for marine biotechnology. September. Taiwan
  7. S. Tsai, W. Yen, C. Lin (2015) Development of cryopreservation techniques for gorgonian oocytes through vitrification. The second conferences on the life sciences & sustainability 2015. May-June. Japan
  8. S. Tsai, P. Thongpooe, C. Lin (2015) Impacts of low temperature preservation on mitochondrial DNA copy number in oocytes of the hard coral Echinopora sp. Low temperature science and biotechnological advances. April. India
  9. C. Lin, W. Yen, S. Tsai (2014) Comparison of propylene glycol and dimethyl sulfoxide based vitrification solutions for the cryopreservation of gorgonian (Junceella juncea) sperm. The asian conferences on the life sciences & sustainability 2014. August. Japan
  10. C. Lin, F.W. Kou, S. Tsai (2014) Membrane lipid phase transition behavior of oocytes from three gorgonian corals in relation to chilling injury. 3rd Asia-pacific coral symposium. June. Taiwan
  11. C. Lin, G. Chong, S. Tsai (2014) Cryopreservation of the symbiotic algae of a gorgonian coral (Junceella fragilis). 10th Asia-pacific marine biotechnology conference. May. Taiwan
  12. C Lin, S Tsai (2014) Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes. 2014 Bangkok international conference on biological engineering & natural science. January. Thailand.
  13. C Lin, S Tsai, Y Jhuang, C.C. Lin (2013) Ultrastructural observation on the oogenesis of gorgonian coral (Junceella juncea). 13TH Asian pacific aquaculture. December. Vietnam.
  14. L.H. Wang, C. Lin, Y.C. Li, Z. Lin, Y.Y. Zhao, C.C. Hu, J.H.Guo, S. Tsai (2013) Relationship between cryobiology and lipid concentration in banded coral shrimp (Stenopus hispidus) embryos. The 5th International symposium for marine biotechnology. September. Taiwan
  15. S Tsai, L.H. Wang, Z. Lin, Y.C. Li, P.J Meng, C. Lin (2013) lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts. International conference on challenges in aquatic science. March. Taiwan
  16. S Tsai, Z. Lin, Y.C. Li, C. Lin (2013). Saving corals by cryopreservation technique. Annual international seminar on marine science and aquaculture. March. Malaysia. (oral presentation)
  17. S Tsai, C. Lin (2012) Development of cryopreservation techniques for coral oocytes. The first Asian marine biology symposium. December. Thailand (oral presentation)
  18. S Tsai, L.H. Wang, Z. Lin, W.C. C, Y.C. Li, C. Lin (2012) Lipid content and composition as an indicator of successful cryopreservation in coral oocytes. The 4th International symposium for marine biotechnology. September. Taiwan
  19. W. Yen, Y. Jhuang, W. Chang, S. Tsai and C. Lin (2011) Attempted cryopreservation of gorgonian (Junceella Juncea) oocytes through vitrification. The 3th International symposium for marine biotechnology. September. Taiwan
  20. Z. Lin, C. Lin and S. Tsai (2011) Assessing the feasibility of using Carassius auratus as feed for the marble goby (Oxyeleotris marmorata) The 3th International symposium for marine biotechnology. September. Taiwan
  21. E. Spikings, C. Lin and S. Tsai (2011) Impact of cryoprotectants on mitochondria of coral oocytes. 29th-30th Research poster exhibition. June. UK
  22. C. Lin and S. Tsai (2009) Development of in vitro culture method for early stage zebrafish (Danio rerio) ovarian follicles for use in cryopreservation studies. The 2th International symposium for marine biotechnology. September. Taiwan
  23. S. Tsai, E .Spiking, F.W. Kuo, N.C. Lin and C. Lin (2009) Use of adenosine triphosphate assay and simultaneous staining with fluorescein diacetate and propidium iodide to evaluate the effect of cryoprotectants on hard coral (Echinopora sp.) oocyte. The 2th International symposium for marine biotechnology. September. Taiwan
  24. S. Tsai and C. Lin (2009) Effects of cryoprotectant on the embryos of banded coral shrimp (Stenopus hispidus), preliminary studies to establish freezing protocols. 46th Annual Meeting of the Society for Cryobiology. July. Japan
  25. C. Lin, E. Spikings, T. Zhang and D.M. Rawson (2009) Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryo and blastomeres 27th-28th Reserch poster exhibition. June. UK
  26. C. Lin, E. Spikings, T. Zhang, D.M. Rawson Evaluation of potential housekeeping genes for use in expression profiling the effects of chilling and freezing in zebrafish embryos and blastomeres. The society for low temperature biology meeting September (2008). Denmark
  27. C. Lin, T. Zhang, G. Elgar, D.M. Rawson. (2008) Cryopreservation and gene expression in zebrafishh blastomere. Cryoletters. 29(1): 85. Abstract.
  28. E. Spikings, C. Lin, T. Zhang, D.M. Rawson. (2008) The effect of chilling on mitochondrial DNA replication in zebrafish embryos. 8th international conference on zebrafish development and genetics. 628. June. USA
  29. C. Lin, E. Spikings, T. Zhang, D.M.Rawson (2008) Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres. 26th-27th Reserch poster exhibition. June. UK
  30. C. Lin, T. Zhang, G. Elgar, D.M. Rawson. (2007) Initial studies on cryopreservation and gene expression in isolated blastomeres of zebrafish (Danio rerio). Cryobiology. 55: 365. Abstract
  31. C. Lin, T. Zhang, G. Elgar, D.M. Rawson (2007) Cryopreservation and gene expression in zebrafishh blastomere. 5th European zebrafish genetics and development meeting. 89. July. The Netherlands

 

其他


 

1. 林家興郭富雯 (2018) 「珊瑚醫院特展」泰國移展紀事。國立海洋生物博物館館訊91

2. 林家興 (2017) 海生館珊瑚醫院特展,新南向前進泰國。國立海洋生物博物館館訊90

3. 林家興 (2015) 珊瑚冷凍方舟—珊瑚礁的未來不是夢。國立海洋生物博物館館訊78

4. 林家興 (2013) 南極海域浮游生物之研究。國立海洋生物博物館館訊

5. 林家興 (2012) 拯救珊瑚礁—低溫冷凍保存技術。科學月刊 511(7), 552-553

6. 方力行郭富雯林家興宋秉鈞王琳麒林嘉瑋何宣慶張祐嘉王自磐 (2012) 零下任務-台灣科學界第一次南極長征。時報文化

7. 林家興 (2011) 珊瑚保育-冷凍基因庫珊瑚世界的探索與了解。國立海洋生物博物館專書48-53

8. 王立雪林家興唐川禾樊同雲陳啟祥 (2011) 珊瑚對溫度變動的生理調適珊瑚世界的探索與了解。國立海洋生物博物館專書29-37

9. 林家興 (2011) 南極企島生態研究。國立海洋生物博物館館訊 9

專利


1. 林家興、方力行、蔡錦玲、蔡淑君、沈朋志 (2009) 水生動物的細胞低溫保存方法 (Method for microdrop cryopreservation of aquatic cells) 中華民國發明專利 I306897

2. 林家興、方力行、蔡淑君 (2006) 引子純化裝置 (A device for oligonucleotide purification and desalting) 中華民國新型專利 M285702

3. 林家興、方力行、王立雪、蔡淑君 (2006) 魚類種苗之孵化裝置及孵化設備 (A system for hatching of aquatic embryos) 中華民國新型專利 M285214

4. 林家興蔡淑君(2009) 蒸氣法冷凍保存裝置 (A device for cryopreservation) 中華民國發明專利 M394447

 

 

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